A guided pipeline for Aligning Patients to Cas

Engineering the Cas-PAM interaction has emerged as an effective protocol for achieving allele-specific targeting, whereas the permissive nature of DNA-RNA interactions alone would not have been sufficient. Such SNV-derived PAM approaches are intended to exploit the formation of PAM sequence solely on the mutated allele, discarding the wild-type allele from cleavage.

In this context, we have developed AlPaCas, to streamline the identification and analysis of SNV-derived PAMs.

With AlPaCas you can:

• Analyze ClinVar-annotated SNVs
• Run structural analyses in the context of selected SNV-derived PAMs
• Customize your search by adding your SNVs, PAM patterns, or structures.

... and more. See the HELP page for further details.