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The
Structurally Conserved Regions (SCRs)
are protein segments that conserve the same main-chain conformation
in all the three-dimensional structures analysed, excluding
the intervening regions whose structure differs markedly amongst
different proteins. The SCRs were presumably subjected to
similar constraints during the divergent evolution of a family
or superfamily of proteins from a common ancestor; therefore
they possibly contain most of the determinants necessary to
maintain the fold. SCR_FIND is a method for
the automatic identification of the SCRs, which assigns a
score for each position of a multiple structure alignment
based on the mean RMSD computed for the correspondent
coordinates of the structurally equivalent alpha carbons.
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Superimposition
of the SCRs found in PLP, fold type I enzymes.
The backbones of 23 superposed structures is shown as
solid oval ribbon. Seventeen regions with a mean positional
RMSD = 3.0 Å, lacking insertions and deletions
were detected and the corresponding coordinates coloured
according to the RMSD value. PLP is displayed as slate
CPKs. Oxygen atoms are coloured red, nitrogen atoms
blue, and phosphorus purple. |
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